Hi, I'm Lingli

Research & Development Professional

Passionate about utilizing the machine learning technique and developing computational workflow to solve Cryo-EM challenges and always eager to collaborate on innovative projects.

Available for opportunities
Lingli Kong

About Me

I’m a computational scientist building physics-aware algorithms and AI tools that turn messy, in-situ cryo-EM data into reliable structure. I’ve contributed multiple C++ programs to the cisTEM suite and related tools (e.g., Unbend for local motion correction, CTFFIND5 tilt components, fit_tilt_model, shift_field_generation), and I develop an orientation-conditioned neural network that augments 2D template matching (2DTM) for membrane-protein detection from liposomes—reducing template bias and enabling one-step reconstructions with minimal additional refinement. My tools are delivered as production-ready C++/Python with reproducible benchmarks. Current focus: a generalizable machine learning model for membrane protein detection from in situ data and calibrated end-to-end pipelines that reduce manual tuning and accelerate 3D reconstructions.

Field of interests

Cryo-EM AI Structural Biology Software Development

Research

Cryo-EM Image Processing

Advanced computational methods for processing cryo-electron microscopy images, focusing on motion correction and contrast transfer function estimation.

Motion Correction CTF Estimation Image Processing
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Deep Learning for Structural Biology

Applying machine learning and deep learning techniques to solve complex problems in cryo-EM data processing and structural analysis.

Template Matching Deep Learning CNN Automation
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Method Development & Software

Developing robust computational tools and software packages that advance the field of structural biology and cryo-EM.

Software Development cisTEM User Interface Open Source
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Projects

Project 1

Membrane Protein Detection from Liposomes using 2DTM and Deep Learning

This project focuses on the detection of membrane proteins from liposome structures using advanced deep learning techniques. Key achievements include the development of a novel 2D template matching algorithm and the successful application of convolutional neural networks for feature extraction.

Python PyTorch 2D Template Matching
Project 2

Local Motion Correction on Cryo-EM Movie Frames

This project focuses on correcting local motion artifacts in cryo-electron microscopy (Cryo-EM) movie frames using advanced image processing techniques. Key achievements include the development of a novel algorithm for motion correction and the successful application of deep learning for artifact removal.

C++ Mathematical Modeling Multicore Processing
Project 3

Deep Learning (CNN-BiLSTM) in Electron Microscopy

A CNN-BiLSTM that learns local edge shapes and longer-range spectral context, trained on simulated + curated experimental spectra.

Electron Energy Loss Spectroscopy (EELS) is powerful but slow and operator-dependent: background subtraction, low SNR, and overlapping edges make peak identification error-prone—especially in high-throughput EM workflows.

CNN feature extractor BiLSTM context head EM

Publications

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Let's Connect

I'm actively seeking new opportunities and would love to discuss how my research and development experience that can contribute to your team.

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